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Amylase is ususally measured in plasma as a test for pancreatitis. Occasionally there may be confusion over the tissue origin of a raised serum amylase when salivary gland or fallopian tubes may be implicated. Ususally the simplest way to determine the likely source is to measure a serum lipase, as this enzyme is not found in the latter two structures. If necessary the pancreatic and salivary forms of the enzyme can be identified by one of two methods. Amylase electrophoresis can determine the predominant form in plasma. This method is not quantitative but can detect Macroamylase. The levels of the two forms can be determined by immune-precipitation using a monoclinal antibody directed against salivary amylase. As each of these methods may take a week before a result is available, lipase is clearly a more useful test in most cases.
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